2d quant kit (Cytiva Europe)

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Cytiva Europe 2d quant kit
2d Quant Kit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 2213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2d+quant+kit/2-D+Quant+kit/pm42152085-108-9-12
Average 96 stars, based on 2213 article reviews

2d quant kit - by Bioz Stars, 2026-10

96/100 stars

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Protein Purification:

Article Title: Proteomics to reveal relationship between menstrual cycle and subjective conditions.
Article Snippet: After depletion of high-abundance proteins, the samples were then concentrated using a Vivaspin 2 MWCO 3000 (Cytiva) to a final volume of 100 μL. .. For protein purification, a 2D clean up kit (Cytiva) was used to remove potential impurities in the sample, and a 2D Quant kit (Cytiva) was used for protein quantification. .. Two-dimensional gel electrophoresis(2-DE) Protein samples were dissolved in DeStreak Rehydration Solution (Cytiva), and 15 μg of protein was loaded onto an Immobiline Drystrip (7 cm, pH 3-10, Cytiva).

Sonication:

Article Title: The structural features and immunological role of biomphalysins in the snail Biomphalaria glabrata
Article Snippet: .. A total protein dosage using 2D-Quant kit (Cytiva, GE80-6483-56) was performed after cell sonication in a lysis buffer (50 mM Tris, 150 mM NaCl, 1% NP-40, 5 mM EDTA), 10 cycles of 30 sec each at 70% intensity at 4°C were performed using a GM mini20 Sonoplus (Bandelin electronics) and a MS1.5 probe. .. For confocal microscopy imaging, sporocysts were fixed in 0.4% paraformaldehyde for 10 minutes and observed through a Zeiss LSM 700 microscope.

Lysis:

Bradford Assay:

Article Title: Chronic activity-based anorexia triggers a glial response in the hippocampus independent of intestinal epithelial toll-like receptor 4.
Article Snippet: Proteins extraction AR TIC LE IN PR ES S Colon proteins were extracted using 2 different lysis buffers adapted to the analysis of the different protein targets studied: buffer A (Hepes pH 7.9 10 mM, KCl 10 mM, MgCl2 1.5 mM, EDTA 0.1 mM), DTT (1 mM), NP40 (0.25%), protease (0.5%) and phosphatase (1%) inhibitors) was used for the study of claudin-1; buffer U-CHAPS (spermine tetrahydrochloride 25 mM, protease (5%) and phosphatase (1%) inhibitors (Sigma-Aldrich), DTT 50 mM (Janssen Pharmaceuticalaan, Geel, Belgium), Urea 8 M (Merck Millipore, Burlington, US), CHAPS 4% (Janssen Pharmaceuticalaan)) for the study of occludin and ZO-1. .. Total protein content was determined using Bradford assay and 2D Quant kit (GE Healthcare, Buc, France), respectively. .. Evaluation of protein expression by western blot Total proteins (20 μg/lane) were separated on 4-20% (claudin-1) or 10% (occludin and ZO-1) gradient polyacrylamide stain-free gel (Bio-Rad Laboratories).

Protein Concentration:

Article Title: Starvation of the bacterium Vibrio atlanticus induces simultaneous attacks on the dinoflagellate Alexandrium pacificum
Article Snippet: .. The protein concentration of the supernatant was estimated using the 2D Quant Kit (Cytiva, MERCK) and samples were stored at –80 °C until use. ..

Article Title: Proteomic Analysis Comparing Effect of Feeding Practices on the Milk Fat Globule Membrane Proteins from Camelus dromedarius
Article Snippet: The resulting soluble protein fraction was purified using a 2D Clean-Up Kit (GE Healthcare, Uppsala, Sweden), and the pH was adjusted to 8.5 with 100 mM NaOH. .. Total protein concentration was determined using the 2D-Quant Kit (GE Healthcare, Chicago, IL, USA). .. Fifty micrograms of protein from each MFGM-DFC ( n = 5) and MFGM-FFC ( n = 5) sample were labeled using the CyDye DIGE minimal labeling protocol (GE Healthcare, Chicago, IL, USA).

Centrifugation:

Article Title: Adverse effects of 6PPD-quinone bioaccumulation at environmentally relevant concentrations on Cyprinus carpio growth and development.
Article Snippet: .. After centrifugation at 10,000 ×g for 10 min at 4 °C, the supernatant was collected, and the protein concentration was measured using the 2D-Quant Kit (GE Healthcare, UK). .. A total of 100 μg protein per sample was reduced, alkylated, and digested with trypsin (Promega, Germany) via in-gel digestion.

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